Rapid assessment of iron in blood plasma and serum by spectrophotometry with cloud-point extraction [version 1; referees: 2 approved]

Rapid photometric assessment of iron in blood plasma and serum by a simple procedure after the extraction of iron(II) complex with 1-nitroso-2-naphthol in the micellar phase of a nonionic surfactant at the cloud point upon heating (pH range is 4.5–6.3) is proposed. The procedure trueness was verified using a standard reference protocol using bathophenanthroline. The advantages of the procedure are higher sensitivity than the reference protocol: the limit of detection is 0.03 μg/mL, the limit of quantitation is 0.1 μg/mL, the determination range is 0.1 – 2.8 μg/mL (RSD 0.02–0.10). Copper does not interfere with the iron assessment. Mikhail Proskurnin ( ) Corresponding author: [email protected] Samarina T and Proskurnin M. How to cite this article: Rapid assessment of iron in blood plasma and serum by spectrophotometry with 2015, :623 (doi: ) cloud-point extraction [version 1; referees: 2 approved] F1000Research 4 10.12688/f1000research.6716.1 © 2015 Samarina T and Proskurnin M. This is an open access article distributed under the terms of the Copyright: Creative Commons Attribution , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Data associated Licence with the article are available under the terms of the (CC0 1.0 Public domain dedication). Creative Commons Zero "No rights reserved" data waiver The work is supported by The Russian Science Foundation, grant no. 14-23-00012 (MP). Grant information: The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing interests: The authors declare that they have no competing interests. 25 Aug 2015, :623 (doi: ) First published: 4 10.12688/f1000research.6716.1 Referee Status: